过氧亚硝酸盐
化学
活性氧
氧化应激
活性氮物种
超氧化物
荧光
光化学
氧化磷酸化
激进的
生物物理学
生物化学
物理
量子力学
生物
酶
作者
Xiao Wang,Xuechuan Wang,Qingxin Han
标识
DOI:10.1016/j.aca.2023.341358
摘要
Mercury is a highly toxic heavy metal pollutant. Mercury and its derivatives pose serious threats to the environment and the health of organisms. Numerous reports have indicated that Hg2+ exposure induces a burst of oxidative stress in organisms, causing severe damage to the health of the organism. A large number of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are produced under conditions of oxidative stress, and superoxide anions (O2-) and NO radicals react rapidly with each other to produce peroxynitrite (ONOO-), an important downstream product. Therefore, developing an efficient and highly responsive screening method to monitor the fluctuations of Hg2+ and ONOO- levels is particularly important. In this work, we designed and synthesized a highly sensitive and highly specific near-infrared probe W-2a, which can effectively detect and distinguish Hg2+ and ONOO- through fluorescence imaging. In addition, we developed a WeChat mini-program called "Colorimetric acquisition" and built an intelligent detection platform to assess the environmental hazards of Hg2+ and ONOO-. The probe can detect Hg2+ and ONOO- in the body through dual signaling, as evidenced by cell imaging, and has successfully monitored fluctuations in the ONOO- levels in inflamed mice. In conclusion, the W-2a probe provides a highly efficient and reliable method for assessing oxidative stress-induced changes in the ONOO- levels in the body.
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