Glucosuria and interference of urinary albumin-to-creatinine ratio

We read with interest the article by Chapman et al.1 regarding the glucosuria interference on the urine albumin-to-creatinine ratio. The authors criticize “the” Jaffe method for assaying creatinine in urine, despite the fact that Jaffe never described a method for measuring creatinine.2 There are several Jaffe assays with their own interference pattern caused by pseudochromogens. Unfortunately, Chapman et al. did not mention the details of their creatinine assay. Urinary creatinine concentrations are generally 2 orders of magnitude higher than the plasma/serum creatinine values, necessitating a 100-fold specimen dilution, making the assay less susceptible to analytical interference.