Tissue elasticity modulates cardiac pacemaker cell automaticity

心脏起搏器 细胞内 弹性(物理) 自动性 生物物理学 缝隙连接 化学 细胞生物学 自愈水凝胶 生物医学工程 材料科学 内科学 生物 医学 神经科学 认知 复合材料 有机化学
作者
Young Hwan Choi,Leng Jing,Jinqi Fan,Rafael J. Ramírez,Hee Cheol Cho
出处
期刊:American Journal of Physiology-heart and Circulatory Physiology [American Physical Society]
标识
DOI:10.1152/ajpheart.00813.2024
摘要

Tissue elasticity is essential to a broad spectrum of cell biology and organ function including the heart. Routine cell culture models on rigid polystyrene dishes are limited in studying the impact of tissue elasticity in distinct regions of the myocardium such as the cardiac conduction system. Gelatin, a derivative of collagen, is a simple and tunable platform for modeling tissue elasticity. We sought to study the effects of increasing tissue stiffness on cardiac pacemaker cell function by employing transcription factor-reprogrammed pacemaker cells cultured on gelatin hydrogels with specific elasticity. Our data indicate that automaticity of the pacemaker cells, measured in rhythmic contractions and oscillating intracellular Ca 2+ transients, was enhanced when cultured on a stiffer matrix of 14 kPa. This was accompanied by increased expression of cardiac pacemaker ion channel, Hcn4, and a reciprocal decrease in Cx43 expression compared to control conditions. Propagation of Ca 2+ transients was slower in the pacemaker cell monolayers compared to control, which recapitulates a hallmark feature in the native pacemaker tissue. Ca 2+ transient propagation of pacemaker cell monolayers was slower on stiffer than on softer hydrogel, and this was dependent on enhanced proliferation of cardiac fibroblasts rather than differences in gap junctional coupling. Culturing the pacemaker cells on rigid plastic plates led to irregular or loss of synchronous contractions as well as unusually long Ca 2+ transient durations. Taken together, our data demonstrate that automaticity of pacemaker cells is augmented by stiffer ECM substrates within the elasticity range of the healthy myocardium. This simple approach presents a physiological in vitro model to study mechanoelectric feedback of cardiomyocytes including the conduction system cells.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
沈静完成签到,获得积分10
2秒前
4秒前
loski发布了新的文献求助10
4秒前
marina完成签到 ,获得积分20
5秒前
8秒前
时尚俊驰发布了新的文献求助10
8秒前
9秒前
文献发布了新的文献求助30
10秒前
13秒前
我嘞个豆完成签到,获得积分10
13秒前
爱笑晓曼发布了新的文献求助10
14秒前
wdy111应助sc采纳,获得20
14秒前
敏感初露发布了新的文献求助10
14秒前
隐形曼青应助机智思真采纳,获得10
17秒前
思源应助时尚俊驰采纳,获得10
17秒前
可爱的函函应助敏感初露采纳,获得10
17秒前
18秒前
爆米花应助橙子采纳,获得10
21秒前
量子星尘发布了新的文献求助10
23秒前
阿满完成签到 ,获得积分10
24秒前
王馨雨完成签到,获得积分10
25秒前
在水一方应助袁涛采纳,获得10
25秒前
爱笑晓曼完成签到,获得积分10
28秒前
29秒前
30秒前
nuoran发布了新的文献求助10
31秒前
31秒前
乐乐宝完成签到,获得积分10
32秒前
33秒前
彭于晏应助阿钉采纳,获得10
34秒前
孙燕应助阿钉采纳,获得10
34秒前
整齐小松鼠应助阿钉采纳,获得10
34秒前
jszhoucl发布了新的文献求助10
35秒前
一定行发布了新的文献求助10
35秒前
jxlu发布了新的文献求助10
36秒前
38秒前
橙子发布了新的文献求助10
38秒前
科研通AI5应助科研通管家采纳,获得10
39秒前
李健应助科研通管家采纳,获得10
39秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989378
求助须知:如何正确求助?哪些是违规求助? 3531442
关于积分的说明 11254002
捐赠科研通 3270126
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173