Experimental and statistical methods for microrheological characterization of heterogeneity in human respiratory mucus mimics of health and disease progression

粘液 表征(材料科学) 疾病 人类健康 统计物理学 材料科学 医学 病理 纳米技术 物理 生物 环境卫生 生态学
作者
Neall Caughman,Micah J. Papanikolas,Matthew R. Markovetz,Ronit Freeman,David B. Hill,M. Gregory Forest,Martin Lysy
出处
期刊:Journal of Rheology [Society of Rheology]
卷期号:68 (6): 995-1011
标识
DOI:10.1122/8.0000789
摘要

Human respiratory mucus (HRM) is extremely soft, compelling passive microrheology for linear viscoelastic characterization. We focus this study on the use of passive microrheology to characterize HRM heterogeneity, a phenomenon in normal HRM that becomes extreme during cystic fibrosis (CF) disease. Specifically, a fraction of the mucin polymers comprising HRM phase-separate into insoluble structures, called flakes, dispersed in mucin-depleted solution. We first reconstitute HRM samples to the MUC5B:MUC5AC mucin ratios consistent with normal and CF clinical samples, which we show recapitulate progressive flake formation and heterogeneity. We then employ passive particle tracking with 200 nm and 1 μm diameter beads in each reconstituted sample. To robustly analyze the tracking data, we introduce statistical denoising methods for low signal-to-noise tracking data within flakes, tested and verified using model-generated synthetic data. These statistical methods provide a fractional Brownian motion classifier of all successfully denoised, tracked beads in flakes and the dilute solution. From the ensemble of classifier data, per bead diameter and mucus sample, we then employ clustering methods to learn and infer multiple levels of heterogeneity: (i) tracked bead data within vs. outside flakes and (ii) within-flake data buried within or distinguishable from the experimental noise floor. Simulated data consistent with experimental data (within and outside flakes) are used to explore form(s) of the generalized Stokes–Einstein relation (GSER) that recover the dynamic moduli of homogeneous and heterogeneous truth sets of purely flakelike, dilute solution, and mixture samples. The appropriate form of GSER is applied to experimental data to show (i) flakes are heterogeneous with gel and sol domains; (ii) dilute solutions are heterogeneous with only sol domains; and (iii) flake and dilute solution properties vary with probe diameter.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI5应助科研通管家采纳,获得10
刚刚
sutharsons应助科研通管家采纳,获得30
刚刚
axin应助科研通管家采纳,获得10
刚刚
terence应助科研通管家采纳,获得30
刚刚
研友_VZG7GZ应助科研通管家采纳,获得10
刚刚
sutharsons应助科研通管家采纳,获得30
刚刚
852应助科研通管家采纳,获得10
刚刚
hh应助科研通管家采纳,获得10
刚刚
sun发布了新的文献求助10
1秒前
1秒前
zhu完成签到,获得积分10
1秒前
酷波er应助缚大哥采纳,获得10
2秒前
李健应助明理雨筠采纳,获得10
2秒前
wang发布了新的文献求助10
4秒前
木头人给step_stone的求助进行了留言
4秒前
魏伯安完成签到,获得积分10
5秒前
朴素尔岚发布了新的文献求助10
6秒前
科研通AI5应助nextconnie采纳,获得10
6秒前
务实的犀牛完成签到,获得积分10
7秒前
7秒前
Blue_Pig发布了新的文献求助10
7秒前
8秒前
科研通AI2S应助橙子fy16_采纳,获得10
9秒前
LGJ完成签到,获得积分10
9秒前
wang完成签到,获得积分10
11秒前
12秒前
13秒前
脑洞疼应助Blue_Pig采纳,获得10
15秒前
16秒前
Akim应助危机的尔蝶采纳,获得10
17秒前
SONG发布了新的文献求助50
17秒前
明理雨筠发布了新的文献求助10
18秒前
小刘一定能读C9博完成签到 ,获得积分10
19秒前
1097完成签到 ,获得积分10
20秒前
缚大哥发布了新的文献求助10
21秒前
Rollei驳回了Hello应助
21秒前
tsntn完成签到,获得积分10
21秒前
wenbo完成签到,获得积分0
21秒前
22秒前
勤奋弋完成签到,获得积分10
25秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Ensartinib (Ensacove) for Non-Small Cell Lung Cancer 1000
Unseen Mendieta: The Unpublished Works of Ana Mendieta 1000
Bacterial collagenases and their clinical applications 800
El viaje de una vida: Memorias de María Lecea 800
Luis Lacasa - Sobre esto y aquello 700
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3527998
求助须知:如何正确求助?哪些是违规求助? 3108225
关于积分的说明 9288086
捐赠科研通 2805889
什么是DOI,文献DOI怎么找? 1540195
邀请新用户注册赠送积分活动 716950
科研通“疑难数据库(出版商)”最低求助积分说明 709849