化学
嘧啶
酶
白色念珠菌
酵母
生物化学
嘧啶代谢
立体化学
氧化磷酸化
微生物学
嘌呤
生物
作者
Anushree Mondal,Rung‐Yi Lai,Dmytro Fedoseyenko,Nitai Charan Giri,Tadhg P. Begley
摘要
The yeast thiamin pyrimidine synthase THI5p catalyzes one of the most complex organic rearrangements found in primary metabolism. In this reaction, the active site His66 and PLP are converted to thiamin pyrimidine in the presence of Fe(II) and oxygen. The enzyme is a single-turnover enzyme. Here, we report the identification of an oxidatively dearomatized PLP intermediate. We utilize oxygen labeling studies, chemical-rescue-based partial reconstitution experiments, and chemical model studies to support this identification. In addition, we also identify and characterize three shunt products derived from the oxidatively dearomatized PLP.
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