The clock gene, period, influences migratory flight and reproduction of the oriental armyworm, Mythimna separata (Walker)

粘虫 生物 交配 繁殖 句号(音乐) 基因 动物 生物钟 昆虫 昼夜节律 细胞生物学 幼虫 遗传学 生态学 内分泌学 物理 声学
作者
Jiayue Ji,Yueqiu Liu,Lei Zhang,Yunxia Cheng,David Stanley,Xingfu Jiang
出处
期刊:Insect Science [Wiley]
卷期号:30 (3): 650-660 被引量:4
标识
DOI:10.1111/1744-7917.13132
摘要

The oriental armyworm, Mythimna separata, is a major long-distance migratory insect pest of grain crops in China and other Asian countries. Migratory flights and reproductive behavior usually occur at night, regulated by a circadian rhythm. However, knowledge about the linkages between adult flight, reproduction, and clock genes is still incomplete. To fill this important gap in our knowledge, a clock gene (designated Msper) was identified and phylogenetic analysis indicated that the encoded protein (MsPER) was highly similar to PER proteins from other insect species. Quantitative RT-PCR assays demonstrated that significantly different spatiotemporal and circadian rhythmic accumulations of mRNA encoding MsPER occurred during development under steady 14 h : 10 h light : dark conditions. The highest mRNA accumulation occurred in adult antennae and the lowest in larvae. Msper was expressed rhythmically in adult antennae, relatively less in photophase and more entering scotophase. Injecting small interference RNA (siRNA) into adult heads effectively knocked down Msper mRNA levels within 72 h. Most siRNA-injected adults reduced their evening flight activity significantly and did not exhibit a normal evening peak of flight activity. They also failed to mate and lay eggs within 72 h. Adult mating behavior was restored to control levels by 72 h post injection. We infer that Msper is a prominent clock gene that acts in regulating adult migratory flight and mating behaviors of M. separata. Because of its influence on migration and mating, Msper may be a valuable gene to target for effective management of this migratory insect.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
田様应助we1light采纳,获得20
1秒前
所所应助100采纳,获得10
2秒前
WHY发布了新的文献求助10
2秒前
天真的秋翠完成签到,获得积分10
2秒前
zhuhan发布了新的文献求助30
3秒前
嘿嘿嘿发布了新的文献求助10
3秒前
4秒前
老实的从菡完成签到,获得积分10
5秒前
大月兔完成签到,获得积分10
6秒前
6秒前
6秒前
研友_Lpa2On发布了新的文献求助10
6秒前
8秒前
Superg完成签到,获得积分10
8秒前
搜集达人应助我很nice采纳,获得10
8秒前
脑洞疼应助石头采纳,获得10
8秒前
8秒前
9秒前
落寞的亦丝关注了科研通微信公众号
9秒前
TYMX完成签到,获得积分10
9秒前
ding应助zhuhan采纳,获得20
9秒前
长期素食发布了新的文献求助30
10秒前
WX完成签到,获得积分10
10秒前
10秒前
qiqi完成签到,获得积分20
10秒前
Ted完成签到,获得积分10
10秒前
10秒前
赘婿应助ceo采纳,获得10
11秒前
11秒前
刘唐荣发布了新的文献求助10
11秒前
ruyinni发布了新的文献求助10
11秒前
Ph发布了新的文献求助10
12秒前
Owen应助xiaowannamoney采纳,获得10
12秒前
苏格拉底的嘲笑完成签到,获得积分10
12秒前
12秒前
机智宛秋完成签到,获得积分10
12秒前
caiyun发布了新的文献求助10
13秒前
我是老大应助jason采纳,获得10
13秒前
小二郎应助坦率耳机采纳,获得30
13秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
An Introduction to Geographical and Urban Economics: A Spiky World Book by Charles van Marrewijk, Harry Garretsen, and Steven Brakman 500
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3151350
求助须知:如何正确求助?哪些是违规求助? 2802831
关于积分的说明 7850478
捐赠科研通 2460184
什么是DOI,文献DOI怎么找? 1309602
科研通“疑难数据库(出版商)”最低求助积分说明 628992
版权声明 601760