苦参碱
细胞凋亡
小RNA
肝细胞癌
癌症研究
上皮-间质转换
转染
下调和上调
细胞生长
转移
化学
生物
细胞培养
医学
癌症
内科学
基因
生物化学
遗传学
色谱法
作者
Baolin Wang,HuiHai Wang,Qin Zhao,Fei Lu,Zhenzhuang Yan,Fang Zhou,Qinglun Su
标识
DOI:10.1080/08977194.2022.2113073
摘要
This study explored the impacts of matrine on hepatocellular carcinoma (HCC) cell growth, metastasis, epithelial-mesenchymal transition (EMT), and stemness through regulating the microRNA (miR)-299-3p/phosphoglycerate mutase 1 (PGAM1) axis. The association between miR-299-3p expression with the prognosis of HCC patients was studied. miR-299-3p and PGAM1 sequences were transfected into matrine-treated HCC cells, and cell proliferation, invasion, apoptosis, and stemness were detected, as well as protein expression of EMT- and stemness-related makers. The targeting relationship between miR-299-3p and PGAM1 was identified. Matrine elevated miR-299-3p expression, repressed proliferation, invasion, and anti-apoptosis of HCC cells, and constrained EMT and stemness in vitro. PGAM1 was a target of miR-299-3p. Repression of PGAM1 rescued the effects of miR-299-3p downregulation on HCC cells. Matrine stimulates HCC cell apoptosis and represses the process of EMT and stemness through the miR-299-3p/PGAM1 axis.
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