适体
生物传感器
化学
检出限
双酚A
胶体金
石墨烯
选择性
吸附
组合化学
纳米颗粒
色谱法
纳米技术
材料科学
有机化学
生物
生物化学
遗传学
催化作用
环氧树脂
作者
Liu-Yin Hu,Jiahua Cui,Yalin Wang,Jinping Jia
出处
期刊:Chemosphere
[Elsevier]
日期:2022-11-06
卷期号:311: 137154-137154
被引量:18
标识
DOI:10.1016/j.chemosphere.2022.137154
摘要
Bisphenol A (BPA) is a harmful endocrine disruptor, sensitive and rapid quantification of BPA is highly desirable. In this work, a novel synergistic signal-amplifying electrochemical biosensor was developed for BPA detection by using a recognition probe (RP) constructed by BPA aptamer modified gold nanoparticles-loaded magnetic reduced graphene oxide (Aptamer-MrGO@AuNPs), and a signal probe (SP) constructed by BPA aptamer-complementary single-stranded DNA (ssDNA) functionalized methylene blue (MB)-loaded gold nanoparticle (ssDNA-AuNP@MBs). The RP and SP can self-assemble to form a stable RP-SP complex through complementary base pairing. The current intensity of the biosensor correlates with the number of RP-SP complexes. In the presence of BPA, the BPA aptamer can capture BPA with high selectivity and affinity, form an RP-BPA complex and dissociate the RP-SP complex to release SP, resulting in a decrease in the current signal intensity of the biosensor. A single AuNP could be loaded with multiple BPA aptamers and MBs, which improves the recognition efficiency and enhances the signal intensity. Due to the magnetic properties of MrGO@AuNPs, the magnetic separation and adsorption of RP or RP-SP complex is very convenient, enabling all reaction processes to be carried out in solution, which not only improves the mass transfer efficiency, but also simplifies the operation. Under optimal conditions, the developed biosensor had a detection limit as low as 0.141 pg/mL and had been successfully applied to the detection of real environmental water samples. Therefore, the synergistic signal amplification strategy of RP and SP has potential value in the detection of trace pollutants in the water environment.
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