免疫系统
材料科学
抗原
小泡
纳米技术
免疫学
医学
生物
生物化学
膜
作者
Yirui Li,Mariela R. Rodriguez-Otero,Julie A. Champion
出处
期刊:Biomaterials
[Elsevier]
日期:2024-06-12
卷期号:311: 122666-122666
标识
DOI:10.1016/j.biomaterials.2024.122666
摘要
Self-assembling protein nanoparticles are beneficial platforms for enhancing the often weak and short-lived immune responses elicited by subunit vaccines. Their benefits include multivalency, similar sizes as pathogens and control of antigen orientation. Previously, the design, preparation, and characterization of self-assembling protein vesicles presenting fluorescent proteins and enzymes on the outer vesicle surface have been reported. Here, a full-size model antigen protein, ovalbumin (OVA), was genetically fused to the recombinant vesicle building blocks and incorporated into protein vesicles via self-assembly. Characterization of OVA protein vesicles showed room temperature stability and tunable size. Immunization of mice with OVA protein vesicles induced strong antigen-specific humoral and cellular immune responses. This work demonstrates the potential of protein vesicles as a modular platform for delivering full-size antigen proteins that can be extended to pathogen antigens to induce antigen specific immune responses.
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