荧光
去甲肾上腺素
神经科学
化学
心理学
多巴胺
光学
物理
作者
Fei Zhang,Yi-Ao Song,Hongzhe Yan,Haifeng Yang,Zihan Liu,Zaifeng Li,Peng Zhang,Caifeng Ding
标识
DOI:10.1016/j.snb.2024.136182
摘要
Norepinephrine (NE) is a neurotransmitter that plays important roles in the monitoring of depression. The significant decrease in its content was positively correlated with the severity of depression. Therefore, the accurate detection of NE is particularly important for tracking the onset of depression. Herein, a lighting up near-infrared (NIR) fluorescent probe Cy-TPC, with both of the excitation and emission wavelength located in NIR region, was designed for the specifically monitor NE. Cy-TPC itself emits almost no fluorescence upon excited at 690 nm. After the incubation with NE, the β-hydroxyethylamine group of NE will attack the carbonate of Cy-TPC, undergo a nucleophilic substitution reaction, and finally release the fluorophore Cy-OH, which leading to the generation of NIR fluorescence at 768 nm. Cy-TPC exhibits high selectivity and acceptable sensitivity (with a LOD calculated as 5.01 μM) for the detection of NE, laying the foundation for its application in biological samples. The subsequent biological imaging experiments indicated that Cy-TPC could be used to monitor the content of NE in PC-12 cells and was successfully applied to the imaging of NE in depressed mice brain.
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