细胞外小泡
纳米技术
材料科学
化学
生物物理学
生物
细胞生物学
作者
Xiaojie Qin,Yuanhang Xiang,Linfeng Mao,Yu Yang,Binqi Wei,Hao Lü,Xinchun Li,Yuanqing Zhang,Fan Yang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2024-05-23
卷期号:18 (22): 14569-14582
被引量:5
标识
DOI:10.1021/acsnano.4c02339
摘要
Accurately assaying tumor-derived circulating extracellular vesicles (EVs) is fundamental in noninvasive cancer diagnosis and therapeutic monitoring but limited by challenges in efficient EV isolation and profiling. Here, we report a bioinspired buoyancy-driven metal-organic framework (MOF) corona that leverages on-bubble coordination and dual-encoded surface-enhanced Raman scattering (SERS) nanotags to streamline rapid isolation and ultrasensitive profiling of plasma EVs in a single assay for cancer diagnostics. This integrated bubble-MOF-SERS EV assay (IBMsv) allows barnacle-like high-density adhesion of MOFs on a self-floating bubble surface to enable fast isolation (2 min, near 90% capture efficiency) of tumor EVs via enhanced EV-MOF binding. Also, IBMsv harnesses four-plexed SERS nanotags to profile the captured EV surface protein markers at a single-particle level. Such a sensitive assay allows multiplexed profiling of EVs across five cancer types, revealing heterogeneous EV surface expression patterns. Furthermore, the IBMsv assay enables cancer diagnosis in a pilot clinical cohort (
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