Flavobacterium soyae sp. nov., isolated from the rhizosphere of soya bean.

A Gram-stain-negative, aerobic, rod-shaped bacterium without flagella, designated SCIV07 T , was isolated from soya bean rhizosphere soil collected from Bazhong, Sichuan Province, PR China and characterized by using polyphasic taxonomy. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCIV07 T belonged to the genus Flavobacterium and most closely related to the type strains Flavobacterium chungangense LMG 26729 T (97.91 %), Flavobacterium reichenbachii LMG 25512 T (97.77 %), Flavobacterium oncorhynchi CCUG 59446 T (97.7 %), Flavobacterium chilense LMG 26360 T (97.7 %) and Flavobacterium plurextorum CCUG 60112 T (97.63 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain SCIV07 T and closely related Flavobacterium species were lower than thresholds of 95 and 70 %, respectively, for species delineation. Catalase and oxidase were positive. Strain SCIV07 T produced flexirubin-type pigments. Menaquinone-6 was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The predominant fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3OH and summed feature 3 (C 16 : 1 ω6 c /C 16 : 1 ω7 c ). Physiological and biochemical features differentiated strain SCIV07 T from the closely related Flavobacterium species. Based on the polyphasic taxonomic data, strain SCIV07 T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium soyae sp. nov. is proposed. The type strain is SCIV07 T (=GDMCC 1.2481 T =JCM 34673 T ).