半抗原
亚甲蓝
免疫分析
色谱法
化学
酶
抗体
生物化学
生物
免疫学
光催化
催化作用
作者
Xuanyun Huang,Guangxin Yang,Yunyu Tang,Liqing Wang
出处
期刊:Chemosensors
[MDPI AG]
日期:2024-03-31
卷期号:12 (4): 52-52
标识
DOI:10.3390/chemosensors12040052
摘要
Methylene blue (MB) is a chemical dye which is used as an alternative drug for malachite green. In this study, based on computational chemistry, a novel hapten (TM) of MB was designed by comparing the conformational and electronic properties of MB. TM was synthesized and further conjugated with bovine serum albumin (BSA) and Ovalbumin (OVA). Then, the polyclonal antibodies were obtained by immunization with the immunogen of TM-BSA. Under optimal conditions, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for the determination of MB, with an IC50 value of 41.5 μg L−1 and displaying a cross reaction of 78.2~88.9% with three different MB metabolins. The limit of detection (LOD) and the limit of quantitation (LOQ) for MB were validated to be 4.8 µg/kg and 6.0 µg/kg, respectively. Spiking experiments showed recoveries between 82.3% and 84.3%, with a relative standard deviation (RSD) of ≤6%. The results showed the generated polyclonal antibodies from the new hapten TM were reliable and could be used for detecting MB in fishery productions.
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