酵母
生物
表达式(计算机科学)
遗传学
基因
计算生物学
基因表达
计算机科学
程序设计语言
作者
Dominik Mojžita,Anssi Rantasalo,Marja Ilmén
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 221-242
被引量:2
标识
DOI:10.1007/978-1-0716-2399-2_13
摘要
The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.
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