Lymphocyte subsets and immunoglobulin levels in peripheral blood from cynomolgus monkeys (Macaca fascicularis) of different age groups

生理学 抗体 年龄组 生物 淋巴细胞 CD8型 免疫学 医学 免疫系统 人口学 社会学
作者
Evgeniya V Voloshina,Yakov Yu Ustyugov,Aleksey A Aleksandrov,Apsuana B Dzheliya,Marina A Oganova
出处
期刊:Laboratory Animals [SAGE Publishing]
卷期号:56 (6): 550-560 被引量:2
标识
DOI:10.1177/00236772221083173
摘要

Immunotoxicity assessment is an important part of non-clinical safety evaluation of biotechnology-derived pharmaceuticals. The reference ranges of evaluated parameters, which depend on the sex, age and geographical origin of animals, play a significant role in interpreting the study results. The aim of this study was to determine the reference ranges of parameters commonly used for non-clinical immunotoxicity studies in cynomolgus monkeys (Macaca fascicularis) of different ages. The percentages of CD3 + , CD4 + , CD8 + , CD20 + , CD16/56 + lymphocytes, and the serum levels of immunoglobulins A, M, G and E in clinically healthy cynomolgus monkeys of both sexes and different ages (37–104 months) were analysed. The lymphocyte subsets were assessed by flow cytometry and serum immunoglobulin levels were determined by enzyme-linked immunosorbent assay. Statistical analysis showed a weak rank correlation of CD3 + , CD20 + lymphocyte levels with the ages of female and male cynomolgus monkeys, and no significant correlation with age for the combined data of both sexes. The serum levels of immunoglobulins A, M, G and E did not have a significant rank correlation with age for the combined data of both sexes, whereas IgA levels in females and IgG levels in males were weakly correlated with age. Overall, these results justify the use of animals aged three to eight years for non-clinical immunotoxicity evaluation of biotechnology-derived pharmaceuticals. It is important to avoid the formation of animal groups with extreme ages (three years or eight years) in one group during randomization.
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