亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Extraction Optimization, Structural Characterization, and Anti-Hepatoma Activity of Acidic Polysaccharides From Scutellaria barbata D. Don

多糖 糖醛酸 阿拉伯糖 化学 木糖 半乳糖 色谱法 鼠李糖 单糖 萃取(化学) 岩藻糖 生物化学 发酵
作者
Wenwen Su,Leilei Wu,Qichao Liang,Xiaoyue Lin,Xiaoyi Xu,Shikai Yu,Yitong Lin,Jiadong Zhou,Fu Yang,Xiaoyan Gao,Bo Zhang,Li Li,Dan Li,Yongkui Yin,Song Gao-chen
出处
期刊:Frontiers in Pharmacology [Frontiers Media SA]
卷期号:13 被引量:11
标识
DOI:10.3389/fphar.2022.827782
摘要

The Chinese medicinal herb Scutellaria barbata D. Don has antitumour effects and is used to treat liver cancer in the clinic. S. barbata polysaccharide (SBP), one of the main active components extracted from S. barbata D. Don, exhibits antitumour activity. However, there is still a lack of research on the extraction optimization, structural characterization, and anti-hepatoma activity of acidic polysaccharides from S. barbata D. Don. In this study, the optimal extraction conditions for SBP were determined by response surface methodology (RSM): the material-liquid ratio was 1:25, the extraction time was 2 h, and the extraction temperature was 90°C. Under these conditions, the average extraction efficiency was 3.85 ± 0.13%. Two water-soluble polysaccharides were isolated from S. barbata D. Don, namely, SBP-1A and SBP-2A, these homogeneous acidic polysaccharide components with average molecular weights of 1.15 × 105 Da and 1.4 × 105 Da, respectively, were obtained at high purity. The results showed that the monosaccharide constituents of the two components were fucose, galactosamine hydrochloride, rhamnose, arabinose, glucosamine hydrochloride, galactose, glucose, xylose, and mannose; the molar ratio of these constituents in SBP-1A was 0.6:0.3:0.6:30.6:3.3:38.4:16.1:8:1.4, and that in SBP-2A was 0.6:0.5:0.8:36.3:4.4:42.7:9.2:3.6:0.7. In addition, SBP-1A and SBP-2A contained uronic acid and β-glucan, and the residue on the polysaccharide was mainly pyranose. The in vitro results showed that the anti-hepatoma activity of SBP-2A was better than that of SBP-1A and SBP. In addition, SBP-2A significantly enhanced HepG2 cell death, as cell viability was decreased, and SBP-2A induced HepG2 cell apoptosis and blocked the G1 phase. This phenomenon was coupled with the upregulated expression of P53 and Bax/Bcl-2 ratio, as well as the downregulated expression of the cell cycle-regulating protein cyclinD1, CDK4, and Bcl-2 in this study. Further analysis showed that 50 mg/kg SBP-2A inhibited the tumour growth in H22 tumour-bearing mice, with an average inhibition rate of 40.33%. Taken together, SBP-2A, isolated and purified from S. barbata showed good antitumour activity in vivo and in vitro, and SBP-2A may be a candidate drug for further evaluation in cancer prevention. This study provides insight for further research on the molecular mechanism of the anti-hepatoma activity of S. barbata polysaccharide.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
CodeCraft应助丢丢采纳,获得10
5秒前
Karol完成签到,获得积分10
7秒前
hanmy完成签到,获得积分10
8秒前
andrele发布了新的文献求助10
12秒前
yangfeidong应助优雅的涵瑶采纳,获得30
12秒前
14秒前
16秒前
黄钰发布了新的文献求助10
21秒前
Nan发布了新的文献求助10
29秒前
优雅的涵瑶完成签到,获得积分20
30秒前
tctgvfxdbhb完成签到,获得积分10
36秒前
38秒前
执着乐双发布了新的文献求助10
47秒前
59秒前
称心的麦片完成签到,获得积分10
59秒前
1分钟前
andrele发布了新的文献求助30
1分钟前
2分钟前
Jerry发布了新的文献求助10
2分钟前
Nefelibata完成签到,获得积分10
2分钟前
CDabin完成签到,获得积分10
2分钟前
2分钟前
英俊的铭应助blue2021采纳,获得10
2分钟前
活力半凡发布了新的文献求助10
2分钟前
2分钟前
Jerry完成签到,获得积分10
2分钟前
房天川完成签到 ,获得积分10
2分钟前
2分钟前
活力半凡完成签到,获得积分20
2分钟前
深情安青应助科研通管家采纳,获得10
3分钟前
Billy应助科研通管家采纳,获得60
3分钟前
hanmy发布了新的文献求助10
3分钟前
LL完成签到,获得积分10
3分钟前
3分钟前
Kevin完成签到,获得积分10
3分钟前
3分钟前
blue2021发布了新的文献求助10
3分钟前
诗筠完成签到 ,获得积分10
3分钟前
3分钟前
ElsaFan完成签到,获得积分10
3分钟前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Effect of reactor temperature on FCC yield 2000
Very-high-order BVD Schemes Using β-variable THINC Method 1020
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Mission to Mao: Us Intelligence and the Chinese Communists in World War II 600
The Conscience of the Party: Hu Yaobang, China’s Communist Reformer 600
MATLAB在传热学例题中的应用 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3303216
求助须知:如何正确求助?哪些是违规求助? 2937559
关于积分的说明 8482383
捐赠科研通 2611428
什么是DOI,文献DOI怎么找? 1425859
科研通“疑难数据库(出版商)”最低求助积分说明 662457
邀请新用户注册赠送积分活动 646943