Anti‐Inflammatory Effects of Opuntia Milpa Alta Polysaccharides Fermented by Lactic Acid Bacteria in Human Keratinocyte HaCaT Cells

哈卡特 活力测定 化学 脂多糖 细胞凋亡 发酵 MTT法 细胞 促炎细胞因子 细胞损伤 肿瘤坏死因子α 炎症 微生物学 药理学 分子生物学 生物化学 生物 免疫学 体外
作者
Yuzhi Zhang,Pingping Li,Shiquan You,Dan Zhao,Quan An,Dongdong Wang,Jiachan Zhang,Meng Li,Changtao Wang
出处
期刊:Chemistry & Biodiversity [Wiley]
卷期号:19 (5) 被引量:5
标识
DOI:10.1002/cbdv.202100923
摘要

Opuntia milpa alta polysaccharides (ODP) are bioactive compounds extracted from Opuntia milpa alta and widely used in the treatment of diseases, but the therapeutic mechanism of ODP on inflammatory injury remains unclear. Therefore, this study explores the effects and mechanisms of ODP in lipopolysaccharide (LPS)-induced inflammation of human keratinocytes (HaCaT). In this experiment, ODP was extracted via the water extraction and fermentation methods, respectively. LPS was then used to induce inflammatory damage in HaCaT cells, and the stimulated cells were treated with different concentrations of ODP. Cell viability was detected by MTT assay, and the concentrations of COX-2, iNOS, IL-6, IL-8, IL-10 and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA). Changes in inflammatory cytokines and related mRNA expression were observed to assess LPS-induced cell damage. In the experiment, it was found that the LPS stimulation of HaCaT cells can induce cellular inflammatory response, reduce cell viability, increase cell apoptosis and increase the expression of COX-2, iNOS, IL-6, IL-8, IL-10 and TNF-α. However, the experimental data shows that ODP can reverse the above results by increasing cell viability, inhibiting cell apoptosis, reducing the expression of the above genes and inactivating antioxidant pathways, which revealed the specific mechanism of ODP repairing LPS-induced inflammatory damage to Hacat cell. In addition, The experimental results showed that fermentation could improve the anti-inflammatory effect of ODP. In conclusion, our experimental results indicate that ODP fermented by lactic acid bacteria can be used as an anti-inflammatory agent.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
瀚的喵发布了新的文献求助30
1秒前
晴朗发布了新的文献求助10
1秒前
SciGPT应助lcy采纳,获得10
1秒前
2秒前
2秒前
wyyyyyyz关注了科研通微信公众号
3秒前
oncoma完成签到 ,获得积分10
3秒前
3秒前
所所应助清风采纳,获得10
3秒前
3秒前
郝宝真发布了新的文献求助10
3秒前
3秒前
4秒前
小李同学完成签到,获得积分10
4秒前
爆米花应助xiaoran采纳,获得10
4秒前
无敌暴龙战神完成签到,获得积分20
4秒前
飘逸楷瑞完成签到,获得积分10
5秒前
刘金龙发布了新的文献求助10
5秒前
huco发布了新的文献求助10
5秒前
satori完成签到,获得积分10
5秒前
兰亭序发布了新的文献求助10
6秒前
6秒前
elevEn完成签到,获得积分10
6秒前
drank发布了新的文献求助10
6秒前
6秒前
Melody完成签到,获得积分10
6秒前
奋斗时光完成签到 ,获得积分10
6秒前
haha完成签到 ,获得积分10
7秒前
yewahch发布了新的文献求助10
7秒前
xona发布了新的文献求助10
7秒前
7秒前
飘逸楷瑞发布了新的文献求助20
8秒前
温柔的老头完成签到,获得积分10
8秒前
小二郎应助bierbia采纳,获得10
8秒前
9秒前
dxwy应助至秦采纳,获得10
9秒前
y彤发布了新的文献求助10
10秒前
10秒前
10秒前
10秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
Foreign Policy of the French Second Empire: A Bibliography 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
XAFS for Everyone 500
Classics in Total Synthesis IV 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3144482
求助须知:如何正确求助?哪些是违规求助? 2796014
关于积分的说明 7817418
捐赠科研通 2452067
什么是DOI,文献DOI怎么找? 1304867
科研通“疑难数据库(出版商)”最低求助积分说明 627330
版权声明 601432