清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Cell-Free DNA as Biomarker for Sepsis by Integration of Microbial and Host Information

基因组 败血症 生物标志物 重症监护室 计算生物学 医学 胎儿游离DNA DNA测序 生物 生物信息学 重症监护医学 内科学 基因 遗传学 胎儿 产前诊断 怀孕
作者
Qiuyu Jing,Chi Hung Czarina Leung,Angela Ruohao Wu
出处
期刊:Clinical Chemistry [Oxford University Press]
卷期号:68 (9): 1184-1195 被引量:18
标识
DOI:10.1093/clinchem/hvac097
摘要

Cell-free DNA (cfDNA) is emerging as a biomarker for sepsis. Previous studies have been focused mainly on identifying blood infections or simply quantifying cfDNA. We propose that by characterizing multifaceted unexplored components, cfDNA could be more informative for assessing this complex syndrome.We explored multiple aspects of cfDNA in septic and nonseptic intensive care unit (ICU) patients by metagenomic sequencing, with longitudinal measurement and integrative assessment of plasma cfDNA quantity, human cfDNA fragmentation patterns, infecting pathogens, and overall microbial composition.Septic patients had significantly increased cfDNA quantity and altered human cfDNA fragmentation pattern. Moreover, human cfDNA fragments appeared to comprise information about cellular oxidative stress and could indicate disease severity. Metagenomic sequencing was more sensitive than blood culture in detecting bacterial infections and allowed for simultaneous detection of viral pathogens. We found differences in microbial composition between septic and nonseptic patients and between survivors and nonsurvivors by 28-day mortality, both on the first day of ICU admission and across the study period. By integrating all the information into a machine learning model, we achieved improved performance in identifying sepsis and prediction of clinical outcome for ICU patients with areas under the curve of 0.992 (95% CI 0.969-1.000) and 0.802 (95% CI 0.605-0.999), respectively.We were able to diagnose sepsis and predict mortality as soon as the first day of ICU admission by integrating multifaceted cfDNA information obtained in a single metagenomic assay; this approach could provide important advantages for clinical management and for improving outcomes in ICU patients.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
卷123发布了新的文献求助10
2秒前
8秒前
糖果苏扬完成签到 ,获得积分10
9秒前
CyrusSo524完成签到,获得积分10
10秒前
有魅力荟发布了新的文献求助10
11秒前
路路完成签到 ,获得积分10
21秒前
scenery0510完成签到,获得积分10
22秒前
南宫清涟完成签到 ,获得积分10
23秒前
27秒前
灿华完成签到 ,获得积分10
30秒前
31秒前
31秒前
35秒前
40秒前
雪花完成签到 ,获得积分10
40秒前
S飞完成签到 ,获得积分10
43秒前
尹静涵完成签到 ,获得积分10
44秒前
jessia发布了新的文献求助10
46秒前
lily完成签到 ,获得积分10
46秒前
快乐的90后fjk完成签到 ,获得积分10
49秒前
自觉石头完成签到 ,获得积分10
49秒前
晶莹黎完成签到,获得积分10
1分钟前
demom完成签到,获得积分10
1分钟前
赘婿应助丰富的晓亦采纳,获得30
1分钟前
新奇完成签到 ,获得积分10
1分钟前
迅速的念芹完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
jessia完成签到,获得积分10
1分钟前
丰富的晓亦完成签到,获得积分10
1分钟前
1分钟前
2分钟前
Wang发布了新的文献求助10
2分钟前
有魅力荟发布了新的文献求助10
2分钟前
三人水明完成签到 ,获得积分10
2分钟前
滕皓轩完成签到 ,获得积分20
2分钟前
神勇的天问完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
Apr9810h完成签到 ,获得积分10
2分钟前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Typology of Conditional Constructions 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3571317
求助须知:如何正确求助?哪些是违规求助? 3141926
关于积分的说明 9444838
捐赠科研通 2843331
什么是DOI,文献DOI怎么找? 1562830
邀请新用户注册赠送积分活动 731326
科研通“疑难数据库(出版商)”最低求助积分说明 718524