胚乳
生物
RNA剪接
选择性拼接
生物发生
基因
小RNA
锌指
遗传学
拼接因子
信使核糖核酸
细胞生物学
作者
Yue Cai,Wenwei Zhang,Yushuang Fu,Zhuangzhuang Shan,Jiahuan Xu,Peng Wang,Fei Kong,Jie Jin,Haigang Yan,Xinyuan Ge,Yongxiang Wang,Xiaoman You,Jie Chen,Xin Li,Weiwei Chen,Xingang Chen,Jing Ma,Xiaojie Tang,Jie Zhang,Yiqun Bao,Ling Jiang,Haiyang Wang,Jianmin Wan
摘要
Amylose content is a crucial physicochemical property responsible for the eating and cooking quality of rice (Oryza sativa L.) grain and is mainly controlled by the Waxy (Wx) gene. Previous studies have identified several Dull genes that modulate the expression of the Wxb allele in japonica rice by affecting the splicing efficiency of the Wxb pre-mRNA. Here, we uncover dual roles for a novel Dull gene in pre-mRNA splicing and microRNA processing. We isolated the dull mutant, du13, with a dull endosperm and low amylose content. Map-based cloning showed that Du13 encodes a C2 H2 zinc-finger protein. Du13 coordinates with the nuclear cap-binding complex to regulate the splicing of Wxb transcripts in rice endosperm. Moreover, Du13 also regulates alternative splicing of other protein-coding transcripts and affects the biogenesis of a subset of microRNAs. Our results reveal an evolutionarily conserved link between pre-mRNA splicing and microRNA biogenesis in rice endosperm. Our findings also provide new insights into the functions of Dull genes in rice and expand our knowledge of microRNA biogenesis in monocots.
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