蛋白质二级结构
Web服务器
生物信息学
生物
反平行(数学)
圆二色性
计算生物学
蛋白质数据库
蛋白质结构
上传
同源建模
结晶学
生物系统
计算机科学
生物信息学
遗传学
物理
生物化学
化学
万维网
基因
互联网
量子力学
磁场
酶
作者
András Micsonai,Éva Moussong,Frank Wien,Eszter Boros,Henrietta Vadászi,Nikoletta Murvai,Young‐Ho Lee,Tamás Molnár,Matthieu Réfrégiers,Yuji Goto,Ágnes Tantos,József Kardos
摘要
Abstract Circular dichroism (CD) spectroscopy is widely used to characterize the secondary structure composition of proteins. To derive accurate and detailed structural information from the CD spectra, we have developed the Beta Structure Selection (BeStSel) method (PNAS, 112, E3095), which can handle the spectral diversity of β-structured proteins. The BeStSel webserver provides this method with useful accessories to the community with the main goal to analyze single or multiple protein CD spectra. Uniquely, BeStSel provides information on eight secondary structure components including parallel β-structure and antiparallel β-sheets with three different groups of twist. It overperforms any available method in accuracy and information content, moreover, it is capable of predicting the protein fold down to the topology/homology level of the CATH classification. A new module of the webserver helps to distinguish intrinsically disordered proteins by their CD spectrum. Secondary structure calculation for uploaded PDB files will help the experimental verification of protein MD and in silico modelling using CD spectroscopy. The server also calculates extinction coefficients from the primary sequence for CD users to determine the accurate protein concentrations which is a prerequisite for reliable secondary structure determination. The BeStSel server can be freely accessed at https://bestsel.elte.hu.
科研通智能强力驱动
Strongly Powered by AbleSci AI