放大器
环介导等温扩增
指数函数
DNA
计算生物学
基因复制
计算机科学
指数增长
生物系统
生物
聚合酶链反应
物理
数学
生物化学
基因
量子力学
数学分析
作者
Changjia Hu,Jie Zhang,Yanwen Jin,Wenjie Ma,Rongxing Zhou,Huan Du,Peng Yang,Xiandeng Hou,Nansheng Cheng,Junbo Chen
出处
期刊:ChemBioChem
[Wiley]
日期:2022-02-02
卷期号:23 (6)
被引量:3
标识
DOI:10.1002/cbic.202100548
摘要
Isothermal exponential amplification technology has rarely been fabricated as a universal sensing platform for the detection of proteins. To broaden their application, we have developed a strategy, named protein-recognition-initiated exponential amplification reaction (PRIEAR) using protein recognition to induce DNA assembly, which converts protein recognition events into ssDNA amplicons and combines two-stage amplification to achieve exponential amplification. Taking advantage of this principle, diverse biomarkers can be quantified at sub-picomolar concentrations in a homogenous manner, making PRIEAR suitable for clinical settings. Therefore, this strategy can expand the powerful isothermal exponential amplification technology to protein targets and thus provide a new toolbox in clinical and biomedical applications.
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