蛋白激酶B
PI3K/AKT/mTOR通路
MMP2型
细胞凋亡
癌症研究
信号转导
MMP9公司
癌细胞
焦点粘着
化学
细胞生物学
癌症
转移
生物
下调和上调
生物化学
遗传学
基因
作者
Jeong Eun Gong,Ji Eun Kim,Su Jin Lee,You Jeong Jin,Young Whan Choi,Sun Choi,Dae Youn Hwang
出处
期刊:Molecules
[MDPI AG]
日期:2022-01-23
卷期号:27 (3): 737-737
被引量:4
标识
DOI:10.3390/molecules27030737
摘要
α-Cubebenoate derived from Schisandra chinensis has been reported to possess anti-allergic, anti-obesity, and anti-inflammatory effects and to exhibit anti-septic activity, but its anti-cancer effects have not been investigated. To examine the anti-cancer activity of α-cubebenoate, we investigated its effects on the proliferation, apoptosis, and metastasis of CT26 cells. The viabilities of CT26 cells (a murine colorectal carcinoma cell line) and HCT116 cells (a human colon cancer cell line) were remarkably and dose-dependently diminished by α-cubebenoate, whereas the viability of CCD-18Co cells (a normal human fibroblast cell line) were unaffected. Furthermore, α-cubebenoate treatment increased the number of apoptotic CT26 cells as compared with Vehicle-treated cells and increased Bax, Bcl-2, Cas-3, and Cleaved Cas-3 protein levels by activating the MAP kinase signaling pathway. α-Cubebenoate also suppressed CT26 migration by regulating the PI3K/AKT signaling pathway. Furthermore, similar reductions were observed in the expression levels of some migration-related proteins including VEGFA, MMP2, and MMP9. Furthermore, reduced VEGFA expression was found to be accompanied by the phosphorylations of FAK and MLC in the downstream signaling pathway of adhesion protein. The results of the present study provide novel evidence that α-cubebenoate can stimulate apoptosis and inhibit metastasis by regulating the MAPK, PI3K/AKT, and FAK/MLC signaling pathways.
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