A facile and sensitive HPLC–MS/MS method for quantification of 3,3′‐diindolylmethane in plasma samples

化学 色谱法 生物分析 蛋白质沉淀 检出限 分析物 甲酸 高效液相色谱法 洗脱 盐析 代谢物 生物化学 物理化学 水溶液
作者
A. P. Lakeev,E. A. Yanovskaya,D. V. Tsuran,O. S. Bryushinina,N. Yu. Abdrashitova,Yu. G. Zyuz’kova,В. В. Удут,В. И. Киселев,И. Н. Кузнецов
出处
期刊:Biomedical Chromatography [Wiley]
卷期号:36 (3) 被引量:2
标识
DOI:10.1002/bmc.5296
摘要

Indole-3-carbinol is the subject of ongoing biomedical research owing to its potential antiatherogenic, anticarcinogenic and antioxidant effects. The antitumor properties are mainly associated with its major metabolite, i.e. 3,3'-diindolylmethane (DIM). Typically, the biological activity of the chemical compound is manifested in the ng/ml concentration range. Consequently, the development of highly sensitive analytical methods to determine DIM in various biological samples is an urgent issue. In this study, an HPLC-MS/MS method was established for the quantification of DIM in human plasma. The developed method was validated according to the European Medicines Agency guidelines. Sensitivity, selectivity, accuracy and precision were good, allowing DIM quantification in the concentration range of 5-500 ng/ml. The limit of detection and the lower limit of quantification were 1 and 5 ng/ml, respectively. 4-Methoxy-1-methylindole was used as an internal standard (IS). The analytes were extracted from the human plasma by the acetonitrile-induced protein precipitation method with the addition of 3 mol/L ammonium sulfate as a salting-out agent, which is a facile and efficient approach for high-throughput bioanalysis. The chromatographic separation was performed on the Synergi Fusion-RP C18 column (50 × 2.0 mm, 4 μm, 80 Å) under isocratic elution at 40°C. The mobile phase consisting of acetonitrile and water (0.1% formic acid; 85:15, v/v) was delivered at a flow rate of 0.20 ml/min. DIM and the IS were eluted at 2.36 ± 0.04 and 2.43 ± 0.03 min, respectively. The total analysis time was 3.20 min. Atmospheric pressure chemical ionization was carried out using multiple reaction monitoring in the positive polarity mode. The ion transitions were set to m/z 247.1 → 130.1 (DIM) and 162.1 → 147.1 (IS). The method was successfully applied to the analysis of plasma samples after a single oral administration of the Indinol® Forto drug (200 mg) to healthy female Russian volunteers. Also, the developed method was used for the analysis of rabbit plasma samples after a single oral dose of DIM (20 mg/kg).
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
大气的玉米完成签到,获得积分10
刚刚
研友_VZG7GZ应助科研小白采纳,获得10
刚刚
gennp完成签到,获得积分10
1秒前
范雅寒完成签到 ,获得积分10
1秒前
fangyuan发布了新的文献求助10
1秒前
桐桐应助阿福采纳,获得30
3秒前
彩色的芝麻完成签到 ,获得积分10
3秒前
4秒前
7秒前
7秒前
7秒前
7秒前
小王发布了新的文献求助10
12秒前
樊丽彤发布了新的文献求助10
12秒前
范炎炎发布了新的文献求助10
12秒前
12秒前
13秒前
14秒前
14秒前
善学以致用应助fangyuan采纳,获得10
14秒前
负责又亦发布了新的文献求助10
17秒前
Vinny发布了新的文献求助10
17秒前
18秒前
junjie完成签到,获得积分10
18秒前
18秒前
LI完成签到 ,获得积分10
18秒前
樊丽彤完成签到,获得积分10
20秒前
小王发布了新的文献求助10
20秒前
wjw发布了新的文献求助10
20秒前
范炎炎完成签到,获得积分10
20秒前
Singularity应助neufy采纳,获得10
21秒前
23秒前
牛马刘发布了新的文献求助10
24秒前
上官若男应助和谐竺采纳,获得10
24秒前
xiangwang发布了新的文献求助10
24秒前
25秒前
26秒前
26秒前
雍雍完成签到 ,获得积分10
26秒前
27秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Fundamentals of Dispersed Multiphase Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3254378
求助须知:如何正确求助?哪些是违规求助? 2896597
关于积分的说明 8293307
捐赠科研通 2565536
什么是DOI,文献DOI怎么找? 1393084
科研通“疑难数据库(出版商)”最低求助积分说明 652418
邀请新用户注册赠送积分活动 629946