Biosynthesis of Himastatin: Assembly Line and Characterization of Three Cytochrome P450 Enzymes Involved in the Post‐tailoring Oxidative Steps

It's only natural: The biosynthetic pathway of himastatin from Streptomyces himastatinicus featuring unusual domain organizations is elucidated by genome scanning, generation of in-frame gene deletion mutants, and complementation experiments. Three cytochrome P450s that catalyze novel post-tailoring oxidative reactions (see picture) are characterized.