Indirect mass spectrometric methods for characterizing and sequencing oligonucleotides

Mass Spectrometry ReviewsVolume 15, Issue 5 p. 297-336 Indirect mass spectrometric methods for characterizing and sequencing oligonucleotides Patrick A. Limbach, Corresponding Author Patrick A. Limbach Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803Search for more papers by this author Patrick A. Limbach, Corresponding Author Patrick A. Limbach Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803Search for more papers by this author First published: 1996 https://doi.org/10.1002/(SICI)1098-2787(1996)15:5<297::AID-MAS2>3.0.CO;2-DCitations: 110AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The use of mass spectrometry for the characterization and sequence determination of oligonucleotides is reviewed. This review focuses primarily on the use of mass spectrometry to analyze sequence-specific fragments of oligonucleotides that are generated via solution-phase chemical reactions. The majority of these “indirect” sequencing methods are a result of recent advances in electrospray ionization and matrix-assisted laser desorption/ionization for the generation of intact gas-phase ions from oligonucleotides. Descriptions of the current indirect sequencing protocols will be presented as well as a comparison of the applicability of these procedures for analyzing “real world” samples. The applicability of indirect mass spectrometric sequencing to antisense oligonucleotides will be discussed in detail. © 1997 John Wiley & Sons, Inc. References 1 Baker, T. R.; Keough, T.; Dobson, R. L. M.; Riley, T. A.; Hasselfield, J. A.; Hesselberth, P. 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