N-乙酰氨基葡萄糖
生物化学
酶
酵母
乙酰氨基葡萄糖
核苷酸糖
尿苷
基质(水族馆)
大肠杆菌
尿苷二磷酸
重组DNA
化学
糖基转移酶
核苷酸
变位酶
生物合成
生物
核糖核酸
基因
生态学
作者
Jiewen Zhou,Limei Fan,Peilian Wei,Lei Huang,Jin Cai,Zhinan Xu
标识
DOI:10.1080/10826068.2010.488518
摘要
Uridine 5′-diphospho N-acetylglucosamine (UDP-GlcNAc) is an important nucleotide sugar in the biochemistry of all living organisms, and it is an important substrate in the synthesis of oligosaccharides. In the present work, three bioactive enzymes, namely, glucokinase (YqgR), GlcNAc-phosphate mutase (Agm1), and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU), were produced effectively as soluble form in recombinant Escherichia coli. These three enzymes and dried yeast together were used to construct a multistep enzymatic system, which could produce UDP-GlcNAc efficiently with N-acetylglucosamine (GlcNAc) as the substrate. After the optimization of various reaction conditions, 31.5 mMUDP-GlcNAc was produced from 50 mMGlcNAc and 50 mMUMP.
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