Estrogen Receptors α and β Are Differentially Expressed in Developing Human Bone1

Estrogen plays an essential role in the development and maintenance of the skeleton; its effects are mediated via interactions with two estrogen receptor (ER) subtypes, α and β. The aim of this study was to establish the cellular distribution of ERα and ERβ in neonatal human rib bone. ERα and ERβ immunoreactivity was seen in proliferative and prehypertrophic chondrocytes in the growth plate, with lower levels of expression in the late hypertrophic zone. Different patterns of expression of the two ERs were seen in bone. In cortical bone, intense staining for ERα was observed in osteoblasts and osteocytes adjacent to the periosteal-forming surface and in osteoclasts on the opposing resorbing surface. In cancellous bone, ERβ was strongly expressed in both osteoblasts and osteocytes, whereas only low expression of ERα was seen in these areas. Nuclear and cytoplasmic staining for ERβ was apparent in osteoclasts. These observations demonstrate distinct patterns of expression for the two ER subtypes in developing human bone and indicate functions in both the growth plate and mineralized bone. In the latter, ERα is predominantly expressed in cortical bone, whereas ERβ shows higher levels of expression in cancellous bone.