The epidemiology of Entamoeba histolytica in a rural and an urban area of Mexico. A pilot survey II

Stocks of intestinal amoebae grown in monoxenic culture, were compared against each other and against those previously reported from Mexico City. These were isolated from subjects in San Cristobal de las Casas, Chiapas (rural area) and hospital patients in Merida, Yucatan (urban area). Electrophoretic patterns of the four enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM), L-malate: NADP+ oxido-reductase (oxalacetate-decarboxylating) (ME) and hexokinase (HK) demonstrated the presence of five groups (∗ Zymodeme is a population of amoebae differing from similar populations in the electrophoretic mobilities of one or more specified isoenzymes.