环介导等温扩增
多重位移放大
DNA
分子生物学
PCR的应用
生物
聚合酶链反应
遗传学
基因
DNA提取
数字聚合酶链反应
作者
Мarianna Soroкa,B Wasowicz,Anna Rymaszewska
出处
期刊:Cells
[MDPI AG]
日期:2021-07-29
卷期号:10 (8): 1931-1931
被引量:123
标识
DOI:10.3390/cells10081931
摘要
In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.
科研通智能强力驱动
Strongly Powered by AbleSci AI