A new magnetic Fe3O4@SiO2@TiO2-APTMS-CPA adsorbent for simple, fast and effective extraction of aflatoxins from some nuts

A novel modified magnetic adsorbent was synthesized by binding 3-aminopropyltrimethoxysilane and 1,8-bis (3-chloropropoxy) anthracene-9,10-dione to magnetic nanoparticles. The adsorbent has been characterized by transmission electron microscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, fourier transform infrared spectroscopy. The extraction parameters of nanoparticle amount, pH, vortex time, and desorption conditions (solvent, volume, time) were optimized by combining aflatoxin (AFB1, AFB2, AFG1, AFG2) analyzes in samples, high-performance liquid chromatography-fluorescence detection method, and post-column derivatization. The proposed method was compared with the conventional immunoaffinity column method. Under optimum conditions, the detection limits (LODs) of the aflatoxins range from 0.05 μg kg−1 to 0.15 μg kg−1. The recovery of the four aflatoxins for the added nut samples ranged from 87.7 % to 97.5 %. In addition, the intra-day and inter-day relative standard deviations were <7.1 %. The satisfactory results show that the synthesized adsorbent has advantages such as good sensitivity and accuracy, environmental friendliness, simplicity, easy of application, reliability, and speed in the extraction of aflatoxins in some nuts.