Early-diagnosis of glioma through fluorescence detection of sEVs surface protein via isothermal cascade amplification strategy

The glioma is the most malignant form of brain cancer and the glioma cells could communicate with tumor microenvironment to tune it for benefits through sEVs based way. Therefore, detection of sEVs surface protein may contribute to the early diagnosis of glioma. We proposed here a isothermal and rapid amplification sEVs surface protein analysis method. In the method, an elegantly designed capture probe which is composed of surface protein specific aptamer and partially complementary blocker. In addition, the method exhibited a favorable detection performance in both quantification of sEVs and surface protein analysis. In summary, the method would be very useful to detect sEVs in point-of-care and thus contribute to the diagnosis and treatment of glioma.