With people's increasing awareness of diseases treatment, the researchers began to focus on drug delivery to the exact site of action at the optimal rate. Some researchers have proved that many nanostructures loaded with drugs are significantly better than conventional nanostructures. However, the materials from which the nanostructure determines its performance. To use it as a pharmaceutical ingredient, it must meet strict safety regulatory standards worldwide. Therefore, people's attention has paid to easily available natural substances. As far as we know, bioactive polysaccharides are excellent candidates for realizing these purposes. To be precise, due to the natural availability of polysaccharides, it has been widely used in the research of Nano-biocarriers loaded with drugs. Based on the above analysis, the nanomaterials developed through the laboratory have great potential for upgrading to market products. Therefore, it is of great significance to review the latest progress of polysaccharide-based Nano-biocarriers in drug delivery and their application in diseases treatment. In this work, we focused on the preparation of polysaccharides-based Nano-biocarriers, commonly used polysaccharides for preparing Nano-biocarriers, and drugs loaded on polysaccharides-based Nano-biocarriers to treat diseases. Shortly, polysaccharide-based Nano-biocarriers will be increasingly used in drug delivery and treatment of diseases.