Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells

British Journal of DermatologyVolume 138, Issue 3 p. 407-411 Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells Lachgar, Lachgar Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Charveron, Charveron Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Gall, Gall Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Bonafe, Bonafe Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Lachgar, Lachgar Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Charveron, Charveron Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Gall, Gall Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author Bonafe, Bonafe Laboratoire de Biologie Cellulaire Cutanée, Institut de Recherche Pierre Fabre, Faculté de Médecine Rangueil, 133, route de Narbonne, 31064 Toulouse, FranceSearch for more papers by this author First published: 04 January 2002 https://doi.org/10.1046/j.1365-2133.1998.02115.xCitations: 156AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The hair follicle dermal papilla which controls hair growth, is characterized in the anagen phase by a highly developed vascular network. We have demonstrated in a previous study that the expression of an angiogenic growth factor called vascular endothelial growth factor (VEGF) mRNA varied during the hair cycle. VEGF mRNA is strongly expressed in dermal papilla cells (DPC) in the anagen phase, but during the catagen and telogen phases, VEGF mRNA is less strongly expressed. This involvement of VEGF during the hair cycle allowed us to determine whether VEGF mRNA expression by DPC was regulated by minoxidil. In addition, the effect of minoxidil on VEGF protein synthesis in both cell extracts and DPC-conditioned medium, was investigated immunoenzymatically. Both VEGF mRNA and protein were significantly elevated in treated DPC compared with controls. DPC incubated with increasing minoxidil concentrations (0.2, 2, 6, 12 and 24 μmol/L) induced a dose-dependent expression of VEGF mRNA. Quantification of transcripts showed that DPC stimulated with 24 μmol/L minoxidil express six times more VEGF mRNA than controls. Similarly, VEGF protein production increases in cell extracts and conditioned media following minoxidil stimulation. These studies strongly support the likely involvement of minoxidil in the development of dermal papilla vascularization via a stimulation of VEGF expression, and support the hypothesis that minoxidil has a physiological role in maintaining a good vascularization of hair follicles in androgenetic alopecia. Citing Literature Volume138, Issue3March 1998Pages 407-411 RelatedInformation