硫代乙酰胆碱
化学
石墨氮化碳
银纳米粒子
荧光
检出限
核化学
纳米颗粒
甲基对硫磷
对硫磷
乙酰胆碱酯酶
材料科学
氮化碳
色谱法
纳米技术
阿切
有机化学
杀虫剂
光催化
催化作用
酶
农学
生物
量子力学
物理
作者
Yuan Li,Dan Cai,Guosheng Yan,Ping Qiu,Xiaolei Wang
标识
DOI:10.1016/j.jpha.2020.04.007
摘要
A highly sensitive and selective method was developed for both UV–vis spectrophotometric and fluorimetric determination of organophosphorus pesticides (OPs). This method used silver nanoparticles (AgNPs) modified with graphitic carbon nitride (g-C3N4). The AgNPs reduced the fluorescence intensity of g-C3N4. Acetylthiocholine (ATCh) could be catalytically hydrolyzed by acetylcholinesterase (AChE) to form thiocholine, which induces aggregation of the AgNPs. This aggregation led to the recovery of the blue fluorescence of g-C3N4, with excitation/emission peaks at 310/460 nm. This fluorescence intensity could be reduced again in the presence of OPs because of the inhibitory effect of OPs on the activity of AChE. The degree of reduction was found to be proportional to the concentration of OPs, and the limit of fluorometric detection was 0.0324 μg/L (S/N = 3). In addition, the absorption of the g-C3N4/AgNPs at 390 nm decreased because of the aggregation of the AgNPs, but was recovered in presence of OPs because of the inhibition of enzyme activity by OPs. This method was successfully applied to the analysis of parathion-methyl in real samples.
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