The effect of ultra-high pressure technology on the extraction of polysaccharides from Huangshan Stone Ear was studied. The effects of solid-liquid ratio, pressure and time on the yield of polysaccharides from umbilicaria were investigated by single factor experiment, and the experiment was optimized by response surface experiment design. Under the conditions of pressure maintaining time of 14.36 min, pressure of 303 MPa and ratio of solid to liquid of 1: 54, the theoretical yield of polysaccharides was12.85±1.32%, 1.18 times as high as that of hot water extraction and 1.39 times as high as that of ultrasonic-assisted extraction. This experiment proved that it is feasible to use ultra-high pressure technology to assist in the extraction of stone ear polysaccharides. Meanwhile, the ultra-high pressure technology also has the advantages of high efficiency, environmental protection and low temperature requirement, which provides a new and efficient method for extraction of polysaccharides from umbilicaria.