Evaluation of a novel sputum processing ReaSLR methodology for improving sensitivity of smear microscopy in clinical samples

医学 灵敏度(控制系统) 显微镜 医学物理学 病理 肺结核 电子工程 工程类
作者
Anita Sharma,Ashwini Agarwal,C.D.S. Katoch,Sourav Sen,R. Gupta
出处
期刊:Medical journal, Armed Forces India [Elsevier BV]
卷期号:78 (3): 277-282 被引量:1
标识
DOI:10.1016/j.mjafi.2020.05.010
摘要

Early case detection is a crucial step in the control of tuberculosis (TB). Sputum smear microscopy is the primary method of TB diagnosis in developing countries. The modified Petroff's method using sodium hydroxide at concentrations ranging between 2% and 4% to digest the specimen is widely used in developing countries. A novel ReaSLR (ReaMetrix's Sputum Liquefying Reagent) methodology has been proposed as a simple, easy, low-cost, and better alternative to conventional methods for sputum processing. This study was undertaken to evaluate the performance of the ReaSLR method of sputum processing in comparison with that of the modified Petroff's method.Early-morning sputum samples were collected. After preparing a direct smear, each sample was divided into two equal halves and processed by both the methods, i.e., modified Petroff's method and ReaSLR method. Direct smears were graded according to Revised National Tuberculosis Control Program grading, and smears prepared after processing by the two different methods were graded according to Center for Disease Control and Prevention grading. Smear microscopy results were compared taking culture results of samples processed by the modified Petroff's method as the gold standard.The rate of smear positivity with the modified Petroff's method (22.22%) was found to be higher than that with direct smear microscopy (13.56%; p = 0.0002) and the ReaSLR method (17.32%; p = 0.04). The modified Petroff's method was found to be 26.76% more sensitive than direct microscopy and 15.59% more sensitive than the ReaSLR method.The ReaSLR method was not superior to the modified Petroff's method for smear microscopy. Although this method was more sensitive than the direct method in smear microscopy, the modified Petroff's method performed much better than the ReaSLR method.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
麋鹿发布了新的文献求助10
刚刚
2秒前
火星上含海完成签到,获得积分10
4秒前
小马甲应助张雯思采纳,获得10
8秒前
41应助张雯思采纳,获得10
8秒前
李健的粉丝团团长应助123采纳,获得10
8秒前
孙燕应助张雯思采纳,获得10
8秒前
打打应助张雯思采纳,获得10
8秒前
情怀应助张雯思采纳,获得10
8秒前
孙燕应助张雯思采纳,获得10
8秒前
Hello应助张雯思采纳,获得10
8秒前
搜集达人应助张雯思采纳,获得10
8秒前
赘婿应助张雯思采纳,获得10
8秒前
今后应助张雯思采纳,获得10
8秒前
量子星尘发布了新的文献求助10
9秒前
麋鹿完成签到,获得积分20
9秒前
赵子完成签到,获得积分10
12秒前
英姑应助会飞的鱼采纳,获得10
13秒前
16秒前
FashionBoy应助勤恳化蛹采纳,获得10
17秒前
19秒前
平淡小白菜完成签到,获得积分10
21秒前
lzx发布了新的文献求助10
22秒前
Jun发布了新的文献求助10
22秒前
隐形曼青应助搞怪的紫易采纳,获得10
23秒前
24秒前
xxddw发布了新的文献求助10
25秒前
大反应釜发布了新的文献求助20
29秒前
123发布了新的文献求助10
29秒前
hugeyoung完成签到,获得积分10
32秒前
32秒前
耳百完成签到,获得积分10
33秒前
35秒前
36秒前
37秒前
38秒前
38秒前
CR7应助越啊采纳,获得20
39秒前
李健应助123采纳,获得10
40秒前
Jun完成签到,获得积分10
41秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989444
求助须知:如何正确求助?哪些是违规求助? 3531531
关于积分的说明 11254250
捐赠科研通 3270191
什么是DOI,文献DOI怎么找? 1804901
邀请新用户注册赠送积分活动 882105
科研通“疑难数据库(出版商)”最低求助积分说明 809174