Glutathione-responsive hydrogel and molecularly imprinted polymer nanospheres: New aspect on cisplatin delivery

In this study, Glutathione-responsive hydrogel, MIPs and their related NIPs were synthesized by precipitation polymerization method. Effect of HEMA/MAA and EGDMA/TRIM as functional monomer and crosslinking agents on size, morphology, drug loading and releasing were studied. BACy as a disulfide cross-linking agent was used in praparation of Glutathione-responsive hydrogel. The size and morphology of MIPs and NIPs were examined by FE-SEM technique. Results showed EGDMA-synthesized MIPs had the smaller size than TRIM-prepared MIPs. In order to measure the capacity of MIPs or NIPs for binding to cisplatin, static and dynamic adsorption experiments were done. The amounts of cisplatin were measured by HPLC method. The binding capacity of drug by MIPs is observed to be higher than NIPs. The EGDMA-synthesized polymers showed a high percentage of adsorption. NIPs were saturated much sooner than MIPs. In vitro release assays of cisplatin were done in PBS at pH = 7.4 and temperature of 37 °C. The results showed that increase of the amount of GSH cause to increase release of the drug from hydrogel.