Function and characterization of novel antioxidant peptides by fermentation with a wild Lactobacillus plantarum 60

A novel wild Lactobacillus plantarum 60 with strong antioxidant activity was isolated from Kefir and identified morphologically and genetically. The goat milk fermented with L60 was characterized with strong DPPH radical scavenging activity, high viable count, satisfactory acidity and good stability after gastrointestinal digestion. Fractions of L60 fermented goat milk were purified through a successive of ultrafiltration, Sephadex and Reverse-phase high-performance liquid chromatography (RP-HPLC) technologies, and evaluated by the DPPH radical assay, hydroxyl free radical assay and Fe2+ chelating ability. Three novel antioxidative peptides were obtained subsequently and identified as VGINYWLAHK, TPEVDKEALEK and DLLER. The application of purified peptides in goat milk powder showed significantly increased antioxidant capacities. Our study provided a roadmap for a more comprehensive description of the antioxidant capacity of goat milk fermented by a novel L. plantarum 60.