Advanced nitrogen removal from mature landfill leachate via partial nitrification-Anammox biofilm reactor (PNABR) driven by high dissolved oxygen (DO): Protection mechanism of aerobic biofilm

A novel partial nitrification-Anammox biofilm reactor (PNABR) operated under high dissolved oxygen (DO) with pre-anoxic - aerobic - anoxic operational mode was developed for efficient denitrogenation from mature landfill leachate. With DO concentration gradually increasing to 4.03 ± 0.03 mg/L, the ammonia oxidation rate (AOR) was enhanced to 25.8 mgNH4+-N/(L h), while nitrite oxidation bacteria (NOB) was inhibited effectively by alternating free ammonia (FA) and oxygen starvation. DO micro-distribution revealed that estimated 1900 μm of aerobic biofilm could protect anammox biofilm underneath from being inhibited by high DO. qPCR analysis further suggested that ammonia oxidation bacteria (AOB) abundance in whole biofilm was 6.12 × 109 gene copies/(g dry sludge), which was twice than found in the floc. Anammox bacteria accounted for 2.39% of total bacteria in whole biofilm, contributing 90.0% to nitrogen removal. Nitrogen removal rate (NRR) and nitrogen removal efficiency (NRE) finally reached 396.6 gN/(m3 d) and 96.1%, respectively.