Remodeling process in bone of aged rats in response to resistance training

下调和上调 内科学 内分泌学 酶谱 骨重建 信使核糖核酸 运行x2 医学 污渍 基质金属蛋白酶 阻力训练 基因表达 化学 基因 生物化学
作者
Gonçalo Carreiro de Farias,Ivo Vieira de Sousa Neto,Vinícius Guzzoni,Graziéle Deriggi Pisani,Carine Royer,Caroline Lourenço de Lima,Francisco de Assis Rocha Neves,Fabio Henrique Bogni,Keico Okino Nonaka,João Luíz Quagliotti Durigan,Heloisa Sobreiro Selistre‐de‐Araújo,Rita de Cássia Marqueti
出处
期刊:Life Sciences [Elsevier BV]
卷期号:256: 118008-118008 被引量:8
标识
DOI:10.1016/j.lfs.2020.118008
摘要

We investigate the effects of RT on the mechanical function, gene, and protein expression of key factors involved in bone remodeling during aging. Male rats of 3 and 21 months of age were randomly allocated into four groups (8 per group): young sedentary (YS), young trained (YT), old sedentary (OS), and old trained (OT). RT was performed three times per week (12 weeks). Bone tenacity and stiffness were measured by biomechanical tests and mRNA levels of COL1A1, MEPE, SOST, OPG, BMP-2, PPAR-y, MMP-2-9-13, and TIMP-1 were evaluated by quantitative PCR. COL1A1 protein and MMP-2 activity were detected by western blotting and zymography assays. Aging increased stiffness, while BMP-2, OPG, COL1A1 and MMP-2 mRNA levels reduced (OS vs YS; p ≤ 0.05). RT increased the tenacity of the femur and reduced PPAR-γ regardless of age (YT vs. YS; OT vs. OS; p ≤ 0.05). RT downregulated SOST mRNA levels only in the OT group (vs. OS group, p ≤ 0.05). RT mitigated the age-associated increase in MMP-9 mRNA levels (p ≤ 0.05). In young animals, upregulation in MEPE, MMP-13, TIMP-1 were observed after RT, as well an increase in COL1A1 protein and MMP-2 activity (p ≤ 0.05). RT improved bone tenacity independent of aging, which is relevant for mechanical function, while, at protein levels, RT upregulated MMP-2 activity and collagen 1 only in young rats. This study highlights the importance of exercise on bone health and identifies specific molecular changes in response to RT. Our findings provide insights into the mechanisms involved in age-related changes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Hairee发布了新的文献求助10
刚刚
momo发布了新的文献求助10
4秒前
称心尔曼完成签到,获得积分10
5秒前
7秒前
9秒前
谷蓝完成签到,获得积分10
9秒前
11秒前
希望天下0贩的0应助Hairee采纳,获得10
12秒前
Rondab应助ali采纳,获得30
13秒前
懒羊羊完成签到,获得积分10
13秒前
好吃完成签到,获得积分20
13秒前
好吃发布了新的文献求助10
16秒前
16秒前
17秒前
18秒前
量子星尘发布了新的文献求助10
18秒前
张雯思发布了新的文献求助10
18秒前
fjm完成签到,获得积分10
18秒前
老实雁蓉完成签到,获得积分10
19秒前
fjm发布了新的文献求助10
20秒前
22秒前
微醺小王发布了新的文献求助10
22秒前
24秒前
zhang发布了新的文献求助10
24秒前
qqq发布了新的文献求助10
25秒前
26秒前
李潇潇完成签到 ,获得积分10
27秒前
Ava应助笑点低方盒采纳,获得10
27秒前
28秒前
28秒前
胡航航发布了新的文献求助10
30秒前
乖猫要努力应助momo采纳,获得10
31秒前
诚心问筠完成签到,获得积分10
31秒前
云泥完成签到,获得积分10
33秒前
33秒前
隐形曼青应助okface采纳,获得10
34秒前
zhang完成签到,获得积分10
35秒前
hopen完成签到 ,获得积分10
36秒前
丘比特应助SS采纳,获得10
38秒前
月亮moon完成签到,获得积分10
38秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989297
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253893
捐赠科研通 3270097
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809158