内质网
荧光
内生
化学
细胞生物学
生物物理学
荧光素
活体细胞成像
部分
绿色荧光蛋白
生物化学
生物
立体化学
细胞
基因
物理
量子力学
作者
Lei Zhou,Zhiqiang Cheng,Ning Li,Yong-Xi Ge,Hong-Xu Xie,Kongkai Zhu,Aiqin Zhou,Juan Zhang,Kaiming Wang,Cheng‐Shi Jiang
标识
DOI:10.1016/j.saa.2020.118578
摘要
Hydrogen sulfide (H2S) as an important signaling biomolecule participates in a series of complex physiological and pathological processes. In situ and rapid detection of H2S levels in endoplasmic reticulum (ER) is of great importance for the in-depth study of its virtual functional roles. However, the ER-targeting fluorescent probe for the detection of H2S in live cells is still quite rare. Herein, a new ER-targeting fluorescent probe (FER-H2S) for detecting H2S in live cells was characterized in the present study. This probe FER-H2S was built from the hybridization of three parts, including fluorescein-based skeleton, p-toluenesulfonamide as ER-specific group, and 2,4-nitrobenzene sulfonate as a response site for H2S. The response mechanism of the probe FER-H2S to H2S is on the basis of the ring-opening and ring-closing processes in fluorescein moiety. Moreover, the probe FER-H2S was successfully used for the imaging of exogenous and endogenous H2S in ER of live cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI