Syringaresinol Inhibits UVA-Induced MMP-1 Expression by Suppression of MAPK/AP-1 Signaling in HaCaT Keratinocytes and Human Dermal Fibroblasts

哈卡特 MAPK/ERK通路 化学 基质金属蛋白酶 细胞生物学 磷酸化 光老化 p38丝裂原活化蛋白激酶 下调和上调 分子生物学 信号转导 生物 生物化学 体外 遗传学 基因
作者
Jung Hwan Oh,Young Hoon Joo,Fatih Karadeniz,Jaeyoung Ko,Chang‐Suk Kong
出处
期刊:International Journal of Molecular Sciences [MDPI AG]
卷期号:21 (11): 3981-3981 被引量:67
标识
DOI:10.3390/ijms21113981
摘要

Ultraviolet (UV) irradiation induces detrimental changes in human skin which result in photoaging. UV-induced intracellular changes cause degradation of extracellular matrix (ECM). UV-stimulated cleavage of collagen in ECM occurs via matrix metalloproteinases (MMPs). (±)-syringaresinol (SYR), a phytochemical which belongs to the lignan group of polyphenols, was investigated for its ability to reverse the UVA-induced changes in human HaCaT keratinocytes and dermal fibroblasts (HDFs) in vitro. Effect of SYR on UVA-induced changes was investigated by production and activation of MMPs and its transcriptional upstream effectors; mitogen-activated protein kinases (MAPKs) and pro-inflammatory mediators. Levels of expression were determined using ELISA, RT-PCR and immunoblotting. UVA irradiation stimulated the production of MMP-1 and inhibited collagen production. SYR treatment suppressed MMP-1 and enhanced collagen production in UVA-irradiated HaCaT keratinocytes and HDFs. SYR repressed the UV-induced phosphorylation of p38, ERK and JNK MAPKs in HaCaT keratinocytes while only suppressing JNK phosphorylation in HDFs. In addition, SYR was able to inhibit UVA-induced production of inflammatory cytokines; TNF-α, COX-2, IL-1β and IL-6. Moreover, SYR suppressed the activator protein-1 (AP-1), a heterodimer of phosphorylated transcription factors c-Jun and c-Fos. SYR-treatment decreased nuclear levels of activated c-Fos and c-Jun as a mechanism to inhibit UVA-induced transcriptional activities leading to MMP-1 production. In conclusion, current results demonstrated that SYR could inhibit UVA-induced upregulation of MMP-1 by suppressing MAPK/AP-1 signaling in HaCaT keratinocytes and HDFs. Therefore, SYR was suggested as a potential compound with antiphotoaging properties against UVA-induced skin aging.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
种草匠完成签到,获得积分10
1秒前
2秒前
阔达的香之完成签到,获得积分10
2秒前
CodeCraft应助Nayvue采纳,获得20
3秒前
3秒前
今后应助Summer采纳,获得30
4秒前
5秒前
6秒前
7秒前
7秒前
ahhh完成签到,获得积分20
8秒前
隐形曼青应助小王同学采纳,获得10
8秒前
zxr发布了新的文献求助10
11秒前
今后应助朴素的曼易采纳,获得10
12秒前
14秒前
hu发布了新的文献求助10
14秒前
15秒前
风趣映秋发布了新的文献求助10
15秒前
15秒前
15秒前
16秒前
16秒前
深秋远塞完成签到,获得积分10
16秒前
科研通AI2S应助heiha采纳,获得30
16秒前
17秒前
17秒前
17秒前
文献互助1发布了新的文献求助10
19秒前
小燕子完成签到,获得积分10
20秒前
Nayvue发布了新的文献求助20
20秒前
CyrusSo524应助小羊采纳,获得10
20秒前
汉堡包应助旸里采纳,获得30
21秒前
22秒前
呆萌问凝发布了新的文献求助10
22秒前
helix给helix的求助进行了留言
22秒前
AU发布了新的文献求助10
23秒前
复杂瑛完成签到,获得积分10
24秒前
wos完成签到 ,获得积分10
25秒前
nicai发布了新的文献求助10
25秒前
dll发布了新的文献求助10
25秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 610
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Time Matters: On Theory and Method 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3561407
求助须知:如何正确求助?哪些是违规求助? 3135053
关于积分的说明 9410808
捐赠科研通 2835483
什么是DOI,文献DOI怎么找? 1558462
邀请新用户注册赠送积分活动 728229
科研通“疑难数据库(出版商)”最低求助积分说明 716729