抄写(语言学)
核糖体
细胞生物学
计算生物学
生物
翻译(生物学)
RNA聚合酶
细胞
真核翻译
延伸系数
核糖核酸
生物物理学
信使核糖核酸
遗传学
基因
哲学
语言学
作者
Francis J. O’Reilly,Liang Xue,Andrea Graziadei,Ludwig Sinn,Swantje Lenz,Dimitry Tegunov,Cedric Blötz,Neil Singh,Wim J. H. Hagen,Patrick Cramer,Jörg Stülke,Julia Mahamid,Juri Rappsilber
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2020-07-31
卷期号:369 (6503): 554-557
被引量:148
标识
DOI:10.1126/science.abb3758
摘要
Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae We combined whole-cell cross-linking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell.
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