自噬
生物
毛囊
内科学
内分泌学
生育率
下调和上调
卵泡发生
男科
卵泡
卵巢
细胞凋亡
胚胎
细胞生物学
胚胎发生
人口
生物化学
基因
社会学
人口学
医学
作者
Ren Watanabe,Sho Sasaki,Naoko Kimura
标识
DOI:10.1093/biolre/ioz179
摘要
Abstract The number of stockpiled primordial follicles is thought to be responsible for the fate of female fertility and reproductive lifetime. We previously reported that starvation in nonsuckling early neonatal mice increases the number of primordial follicles with concomitant autophagy activation, suggesting that autophagy may accelerate the formation of primordial follicles. In this study, we attempted to upregulate the numbers of primordial follicles by administering an autophagy inducer and evaluated the progress of primordial follicle formation and their fertility during the life of the mice. To induce autophagy, mice were intraperitoneally injected with the Tat-beclin1 D-11 peptide (0.02 mg/g body weight) at 6–54 h or 60–84 h after birth. In animals that received Tat-beclin 1 D-11 by 54 h after birth, the primordial follicle numbers were significantly increased compared with the control group at 60 h. The ratio of expressed LC3-II/LC3-I proteins was also significantly greater. The numbers of littermates from pregnant females that had been treated with Tat-beclin 1 D-11 were maintained at remarkably greater levels until 10 months old. These results were supported by an abundance of primordial follicles at even 13–15 months old.
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