TLR7型
THP1细胞系
单核细胞
流式细胞术
刺激
细胞生物学
化学
巨噬细胞
免疫系统
细胞因子
分子生物学
生物
免疫学
细胞培养
Toll样受体
先天免疫系统
体外
内分泌学
生物化学
遗传学
作者
Hock‐Liew Eng,Yuan‐Ying Hsu,Tsun‐Mei Lin
标识
DOI:10.1016/j.bbrc.2018.02.079
摘要
The recognition of single-stranded RNA by TLR7/8 leads to the production of NF-κB-mediated cytokines and type I IFNs. However, the role of TLR7/8 activation in monocytes and macrophages is still unclear. The aim of this study was to investigate the differences in the activation of TLR7/8 between these two cell types. Microarray analysis, qRT-PCR and flow cytometry were used to analyse TLR7/8 signalling pathways in monocytes and macrophages after stimulation with agonists. Our data indicated that TLR8 agonists activated the NF-κB- and IRF-mediated pathways in THP-1 cells, whereas TLR7 agonists did not. However, silent TLR8 and enhanced TLR7 expression could increase TLR7-induced NF-κB activation in monocytes. TLR7 and TLR8 agonists induced NF-κB activation but no ISG response in PMA-differentiated THP-1 cells. The mRNA levels of pro-inflammatory cytokine were elevated upon CL075 stimulation in macrophages compared to monocytes. Thus, TLR7 and TLR8 might modulate different immune responses in monocytes and macrophages.
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