Effects of Pyracantha Fortuneana Polysaccharide on Scavenging Oxygen Free Radical and Anti-lipid Peroxidation

Objective To investigate the effects of Pyracantha fortuneana polysaccharide on scavenging oxygen free radical and anti-lipid peroxidation.Methods Superoxide anion free radical(O2-·) and hydroxyl free radical(OH·) were induced with auto-oxidation of pyrogallol and Fenton system,then the effects of Pyracantha fortuneana polysaccharide on scavenging O2-·and OH· were determined.The lipid peroxidation was induced with Fe2+-H2O2 system in liver homogenate of healthy rats and CCL4-induced acute hepatic injury rats,then the effects of Pyracantha fortuneana polysaccharide on anti-lipid peroxidation were determined,moreover,the production of lipid peroxidation malondialdehyde(MDA) was measured by thiobarabituric acid(TBA) colorimetric method.Results Pyracantha fortuneana polysaccharide could significantly scavenge O2-·and OH· and inhibit lipid peroxidation of healthy rat liver homogenate induced by Fe2+-H2O2 system.The different dosage of Pyracantha fortuneana polysaccharide could significantly inhibit the lipid peroxidation of liver homogenate from acute hepatic injury rats induced with CCL4 in vitro(P0.01 and/or P0.05),and decrease the contents of MDA in liver(P0.01 and/or P0.05).Conclusion Pyracantha fortuneana polysaccharide possesses the effects of scavenging oxygen free radical and anti-lipid peroxidation.