Expression of Lipid Catabolism Genes in Diannan Small-ear Pigs with Different H-FABP Genotypes

This study aimed to investigate the expression of the lipid metabolism related genes,and the association between the lipid metabolism related genes and triglyceride(TG) content in intramuscular fat cells of Diannan Small-ear pigs with different H-FABP genotypes.The kit was used to determine the triglyceride(TG) content and to investigate the level of mRNA expression of lipid catabolism genes by reverse transcription quantitative real-time PCR(RT-qPCR).The results showed that significantly higher expression levels of carnitine palmitoyl transferase 1(CPT-1),lipoprotein lipase(LPL) and preoxisome proliferator-activated receptor γ(PPARγ) mRNA were found in the HH genotype individuals comparing with that in hh genotype individuals(P0.05).The expression levels of CPT-1,LPL,PPARγ genes and TG content in intramuscular adipocytes were positively correlated.The expression of fat catabolism related genes in Diannan Small-ear pigs with different H-FABP genotypes is discrepant,the expression level of the pig with HH genotype is higher.As the lipid metabolic activity is stronger,this may increase intramuscular fat deposition relatively.