腺相关病毒
重组DNA
基因传递
薄壁组织
分布(数学)
转导(生物物理学)
中枢神经系统
遗传增强
重组病毒
医学
生物
病毒学
病理
基因
神经科学
载体(分子生物学)
生物物理学
生物化学
数学分析
数学
作者
Kevin Nash,Marcia N. Gordon
出处
期刊:Methods in molecular biology
日期:2015-11-26
卷期号:: 285-295
被引量:6
标识
DOI:10.1007/978-1-4939-3271-9_21
摘要
Recombinant adeno-associated virus (rAAV) has become an extremely useful tool for the study of gene over expression or knockdown in the central nervous system of experimental animals. One disadvantage of intracranial injections of rAAV vectors into the brain parenchyma has been restricted distribution to relatively small volumes of the brain. Convection enhanced delivery (CED) is a method for delivery of clinically relevant amounts of therapeutic agents to large areas of the brain in a direct intracranial injection procedure. CED uses bulk flow to increase the hydrostatic pressure and thus improve volume distribution. The CED method has shown robust gene transfer and increased distribution within the CNS and can be successfully used for different serotypes of rAAV for increased transduction of the mouse CNS. This chapter details the surgical injection of rAAV by CED into a mouse brain.
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