Studies on Alkaline Solution Extraction of Polysaccharide from Silkworm Pupa and Its Immunomodulating Activities

The extraction of polysaccharide from silkworm pupa by alkaline solution was studied.The optimum conditions were obtained by using orthogonal design L9(34).The result showed the optimum conditions were as follows:concentration of alkaline solution 0.02 mol·L-1,temperature 80 ℃,time 3 hours.Under these conditions,the contents of total polysaccharide,protein and amino acids in extractives were 27.9%,38.1% and 35.85% respectively. The immunological test in mice showed the polysaccharide of silkworm pupa(PSP) could increase significantly macrophage phagocytosis,enhance remarkably hemolysin antibody and lymphocyte transformation,which indicated PSP had evident nonspecific,cellular and humoral immunity.