Optimization of Methods on Protein Extraction and SDS-PAGE in Chinese Jujube

[Objective] The aim was to optimize the protein extraction method of Chinese jujube leaves and its SDS-PAGE single dimensional electrophoresis conditions.[Method] 5 protein extraction methods and factors affecting the SDS-PAGE single dimensional electrophoresis were compared and optimized in Chinese jujube.[Result] The protein extracted by modified acetone settling process has less impurities and more limpid strap number,meanwhile,a set of Chinese jujube protein detection method of SDS-PAGE gel preparation and its electrophoresis was identified.[Conclusion] This study prepared the technical ground for the further research of interaction mechanism between Chinese jujube and jujube witches broom phytoplasma from the perspective of molecular biology research.